2 Days Workshop On Transcriptome : RNA seq - rasalsi

2 Days Workshop On Transcriptome : RNA seq

Start Date: 7th to 8th Dec 2018

Know About Next Generation Sequencing Data Analysis : RNA seq

Our 2 day comprehensive workshop on “NGS Data Analysis: RNA seq ” aims at providing systematic Hands-on-Training on using advanced Data Analysis application/tools. This workshop had been conceptualized by eminent scientist having substantial experience in the field of Data Analysis.

Topics to be covered in workshop

  • Introduction to sequencing technologies from a data analysts view

  • Common NGS data analysis issues

  • Applications of sequencing technologies

  • Introduction to file formats (e.g. FASTQ, VCF, SAM, BAM) and databases (e.g. SRA, ENA)

  • Preprocessing of raw reads: quality control (FastQC), adapter clipping, quality trimming

  • Introduction to read mapping (Alignment methods, Mapping heuristics)

  • Read mapping (BWA, Bowtie2, STAR)

  • Usage of important NGS toolkits (samtools, BEDtools)

  • Mapping statistics

  • Visualization of mapped reads (IGV, UCSC)

  • Understanding the transcriptome data and how its genenrated

  • Analyzing the transcriptomic data to understand gene expression and differential gene expression

  • Introduction to ChIP-seq analysis with Galaxy

Salient Features

Introduction to Galaxy platform and walk through a multi-step next generation sequencing (NGS) data analysis, starting with quality control.

Review common choices in NGS data analysis, and demonstrate them within the context of Galaxy, taking full advantage of Galaxy’s rich tool set and visualization capabilities.

Brief overview of what is needed to set up your own local Galaxy instance. Galaxy is an open-source, NIH-funded project.

Practical Applications

  • Understand the Next generation sequencing

  • Discuss the advantages and limitations of the sequencing technologies

  • Learn how to use Galaxy server to analyze the NGS data

  • Deal with the raw Illumina data with quality evaluation and filtering

  • Map reads to the reference genomes, call SNPs and predict their phenotype consequence

  • Alignment to a reference, de-novo assembly, SNP and structural variant-calling of next generation DNA and RNA data

  • Use some bioinformatics software and tools to aid with the analysis of NGS data

  • Understanding Gene expression and Differential gene expression

  • Visualize your data in genome browsers and make conclusions

  • Participants will receive workshop training certificate.

Relax !!!!

You are eligible.

Any one from:

Students, fresher’s, Working professional, Experienced Scientists /Research fellows (National Laboratories, Universities & other R&D institutions) and  students

Any Bachelor’s or Master’s like:

BSc, MSc, Btech, Mtech. B.Pharm, M.Pharm, BE, ME

Any Specialization:

Biotechnology, Botany, Zoology, Biochemistry, Microbiology, Genetics, Bioinformatics, Biomedical sciences, Chemistry, Pharmaceutical Sciences, Computational biology, computational chemistry, Molecular biology, cell biology and other life science areas.

Tools & Software's To Be Used In Workshop

  • BWA

  • Bowtie2

  • STAR

  • SAM/BAM format

  • Samtools

  • BEDtools

  • HTcount

  • DEseq



  • NCBI

  • EMBL

  • Galaxy

Batch and Deadline for Registrations

It’s limited seats! Hurry up

RegistrationDates for RegistrationStudentProfessor/ ResearcherNRI/Foreigner
Early-birdTill 10th Nov 20183500/-4500/-5500/-
Standard10th Nov to 7th Dec 2018 4500/- 5500/-6500/-

*Discount of Rs. 500 each if registered in a group of 2 or more

All payment methods are accepted here.

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Term And Conditions

  • Accommodation assistance for out- station candidates will be provided.
  • Id proof required at the time of registration.
  • Fees are non transferable & non refundable in any case.
  • Lunch  & snacks will be provided.


RASA Life Science Informatics,
4th floor.46/10 shakuntal,
Law college Road,Erandwane 411004
For More details contact us on
Phone No. 020-65600408/ 7875793891/ 7038770985/7798668315


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Top Queries

Will I be receiving a certificate/diploma after completion of the training?

RASA will issue an Industry recognized training certificate for the module you have undergone the training. This certificate will be your passport to newer and better opportunities in the life science informatics domain.

What If I happen to miss one or more classes?

In case if you miss one or more classes, you will be provided with backup classes. You can take the backup classes without having to pay anything extra.

What is the fee for training program at RASA?

RASA offers different course modules with varying duration. The fees of the training depend upon the module selected and the duration of the training.

What kind of salary would I be offered in life science domain?

An average salary for a person working in life science and life science informatics starts from INR 2.8-3.5 lakhs per year.

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