3 Days Workshop On “NEXT GENERATION SEQUENCING DATA ANALYSIS: Variant Calling, RNAseq & ChIPseq”

Start Date: 8th to 10th March 2019

Know About NGS Data Analysis: Variant Calling, RNA seq, CHIP-seq

Our 3 day comprehensive workshop on “NGS Data Analysis: Variant Calling, RNAseq, ChIPseq ” aims at providing systematic Hands-on-Training on using advanced Data Analysis application/tools. This workshop had been conceptualized by eminent scientist having substantial experience in the field of Data Analysis.

Topics to be covered in NGS Data Analysis workshop

  • Introduction to sequencing technologies from a data analysts view

  • Common NGS data analysis issues

  • Applications of sequencing technologies

  • Introduction to file formats (e.g. FASTQ, VCF, SAM, BAM) and databases (e.g. SRA, ENA)

  • Preprocessing of raw reads: quality control (FastQC), adapter clipping, quality trimming

  • Introduction to read mapping (Alignment methods, Mapping heuristics)

  • Read mapping (BWA, Bowtie2, STAR)

  • Usage of important NGS toolkits (samtools, BEDtools)

  • Mapping statistics

  • Visualization of mapped reads (IGV, UCSC) 

  • Variant Calling to detect mutations

  • Analyzing the transcriptomic data to understand gene expression and differential gene expression

  • Introduction to ChIP-seq analysis with Galaxy

Salient Features

Understand the Next generation sequencing

Discuss the advantages and limitations of the sequencing technology

Learn how to use Galaxy server to analyze the NGS data

Deal with the raw NGS data with quality evaluation and filtering

Map reads to the reference genomes, call SNPs and predict their phenotype consequence

Alignment to a reference, counting reads for RNA sample data via NGS tools

Major applications in whole exome sequence and RNA sequence data

Understand the importance of ChIP-Seq and its applications

Using bioinformatics software and tools to aid with the analysis of NGS data

Practical Applications

  • Understand the Next generation sequencing

  • Discuss the advantages and limitations of the sequencing technologies

  • Learn how to use Galaxy server to analyze the NGS data

  • Deal with the raw Illumina data with quality evaluation and filtering

  • Map reads to the reference genomes, call SNPs and predict their phenotype consequence

  • Alignment to a reference, de-novo assembly, SNP and structural variant-calling of next generation DNA and RNA data

  • Use some bioinformatics software and tools to aid with the analysis of NGS data

  • Understanding Gene expression and Differential gene expression

  • Visualize your data in genome browsers and make conclusions

  • Participants will receive workshop training certificate.

Relax !!!!

You are eligible.

Any one from:

Students, fresher’s, Working professional, Experienced Scientists /Research fellows (National Laboratories, Universities & other R&D institutions) and  students

Any Bachelor’s or Master’s like:

BSc, MSc, Btech, Mtech. B.Pharm, M.Pharm, BE, ME

Any Specialization:

Biotechnology, Botany, Zoology, Biochemistry, Microbiology, Genetics, Bioinformatics, Biomedical sciences, Chemistry, Pharmaceutical Sciences, Computational biology, computational chemistry, Molecular biology, cell biology and other life science areas.

Tools & Software's To Be Used In Workshop

  • Gene Database

  • GeneMark

  • GenScan

  • SAM/BAM format

  • Bowtie 2

  • BWA

  • Freebayes

  • wANNOVER

  • RNA-Star

  • HT-Seq2

  • DeSeq

  • Deep Tools

  • Galaxy

Batch and Deadline for Registrations

It’s limited seats! Hurry up

Registration Dates for Registration Student Professor/ Researcher Working Professional NRI/ Foreigner
Early-bird Till 15th Feb 2019 3500/- 4000/- 4500/- 5500/-
Standard 11th Feb to 7th March 2019 4000/- 4500/- 5500/- 6500/-

All payment methods are accepted here.

Enquire Here For Workshop

Term And Conditions

  • Accommodation assistance for out- station candidates will be provided.
  • Id proof required at the time of registration.
  • Fees are non transferable & non refundable in any case.
  • Lunch  & snacks will be provided.

Venue

RASA Life Science Informatics,
4th floor.46/10 shakuntal,
Law college Road,Erandwane 411004
For More details contact us on
Phone No. 020-65600408/ 7875793891/ 7038770985/7798668315

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RASA will issue an Industry recognized training certificate for the module you have undergone the training. This certificate will be your passport to newer and better opportunities in the life science informatics domain.

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In case if you miss one or more classes, you will be provided with backup classes. You can take the backup classes without having to pay anything extra.

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RASA offers different course modules with varying duration. The fees of the training depend upon the module selected and the duration of the training.

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