Courses

Primer Designing By Primer Blast Primers serve a crucial function in identifying the target area of the complex genome [1]. Primers or oligonucleotides are short sequences of DNA or RNA that are complementary to a particular DNA sequence, allowing replication to occur during a PCR cycle. Primers may also be used in various techniques, including […]

CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR associated), an immune system in bacteria and archaea that targets the nucleic acids of viruses and plasmids, is currently frequently employed as a genome editing tool due to its ease of use and effectiveness [1–5]. Using its single-guide RNA (sgRNA), the most common endonuclease, type II […]

High-throughput sequencing (HTS) is a powerful discovery to screen for non-specific germ line variants, somatic mutations and structural variants, profile copy number variations in cancers [1], assemble genomes of microbial organisms [2][3], quantify gene expression [4], identify cell populations from single-cell transcriptomes in a variety of tissues [5], and track epigenetic changes in developing organisms […]

DeepMind’s artificial intelligence system AlphaFold2, predicts the 3D structure of a protein based on its amino acid sequence. It looks to be a 50-year-old answer to the problem of protein folding. DeepMind’s AlphaFold1 was the first in the series, and it scored high in the 13th Critical Assessment of Protein Structure Prediction (Deepmind’s first effort […]

RNA sequencing (RNA-seq) is a genomic approach for the detection and quantitative analysis of messenger RNA molecules in a biological sample and is useful for studying cellular responses. scRNA-seq can describe RNA molecules in individual cells with high resolution and on a genomic scale. scRNA-seq is a relatively new technology first introduced by Tang et […]

A number of human genetic disorders cause chromosomal breakage, which is characterized by genome instability. Genome instability is associated with immune deficiency, a predisposition to develop cancer, and premature aging . Mutations in the BLM or RECQL3 gene located on chromosome 15 which encodes a DNA helicase in the RecQ family cause Bloom syndrome. The RecQ family is […]

Application of NGS in PCOS Study: Polycystic ovary syndrome (PCOS) is a highly complex endocrine disorder in reproductive females, which leads to reproductive, metabolic and hormonal abnormalities and manifest a host of ovarian defects like impaired folliculogenesis, anovulation, and poor oocyte quality. Addressing the epigenetic anomalies that regulate adverse events is of foremost importance in […]

Molecular biology is the study of biomolecules like DNA, RNA and protein. The area interknits with the other areas of biology, Chemistry, Biochemistry and Genetics. However, Biochemistry is the study of chemical molecule and vital processes occurring in organisms. Biochemists mainly focus on role, functions and structure of biomolecule. Genetics is the study of effect […]

  NGS is a low-priced, speedy and high-throughput alternative to Sanger-method based DNA sequencing. It offers parallel-sequencing of millions of DNA fragments concurrently and allows an entire genome to be sequenced in a small time. NGS is basically a three-step process, namely: 1). Template preparation 2). Sequencing and imaging 3). Data analysis. All the platforms […]

Since the beginning of the Human Genome Project, data management has been perceived as a key challenge for current molecular biology research. Before the finish of the nineties, advances had been set up that adequately bolstered most continuous activities, ordinarily based upon relational database management frameworks. Ongoing years have seen a sensational increment in the […]