Next Generation Sequencing

CRISPR Web-based editing tool - CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR associated), an immune system in bacteria and archaea that targets the nucleic acids of viruses and plasmids, is currently frequently employed as a genome editing tool due to its ease of use and effectiveness [1–5]. Using its single-guide RNA (sgRNA), the [...]

High-throughput sequencing (HTS) is a powerful discovery to screen for non-specific germ line variants, somatic mutations and structural variants, profile copy number variations in cancers [1], assemble genomes of microbial organisms [2][3], quantify gene expression [4], identify cell populations from single-cell transcriptomes in a variety of tissues [5], and track epigenetic changes in developing organisms […]

RNA sequencing (RNA-seq) is a genomic approach for the detection and quantitative analysis of messenger RNA molecules in a biological sample and is useful for studying cellular responses. scRNA-seq can describe RNA molecules in individual cells with high resolution and on a genomic scale. scRNA-seq is a relatively new technology first introduced by Tang et […]

A number of human genetic disorders cause chromosomal breakage, which is characterized by genome instability. Genome instability is associated with immune deficiency, a predisposition to develop cancer, and premature aging . Mutations in the BLM or RECQL3 gene located on chromosome 15 which encodes a DNA helicase in the RecQ family cause Bloom syndrome. The RecQ family is […]

  NGS AND PCOS Genetics Study: To start with Polycystic ovary syndrome (PCOS), it is a highly complex endocrine disorder in reproductive females, which leads to reproductive, metabolic, as well as hormonal abnormalities and manifests a host of ovarian defects like impaired folliculogenesis, anovulation, and poor oocyte quality. Moreover Addressing the epigenetic anomalies that regulate [...]

  NGS is a low-priced, speedy and high-throughput alternative to Sanger-method based DNA sequencing. It offers parallel-sequencing of millions of DNA fragments concurrently and allows an entire genome to be sequenced in a small time. NGS is basically a three-step process, namely: 1). Template preparation 2). Sequencing and imaging 3). Data analysis. All the platforms [...]

Since the beginning of the Human Genome Project, data management has been perceived as a key challenge for current molecular biology research. Before the finish of the nineties, advances had been set up that adequately bolstered most continuous activities, ordinarily based upon relational database management frameworks. Ongoing years have seen a sensational increment in the [...]

Essentially, Perl is an interpreted programming language that is easily grasped by newcomers in the bioinformatics field. Additionally, it not only connects software applications together into sequence analysis pipelines, but it also converts file formats and extracts information from the output of analyzed programs. Moreover, Perl/Bio-Perl: A Bioinformatics toolkit is a collection of Perl modules [...]

Genome-wide examinations concerning cooperative interactions among genomic functions, e.g. DNA replication, isolation, translation, repair and rearrangement, are fundamental for methodically explaining every single biological activity on the genome. To this end, chromatin immunoprecipitation pursued by sequencing (ChIP-seq) examination was produced to comprehend the collaboration and cooperations that happen in a wide assortment of living beings […]

With the recent advancement in DNA sequencing technologies, researches have been fortified to high-throughput, cost-efficient sequencing of many more genomes that was ever previously possible. The most prominent area of genomic sequencing and assembly, known as de-novo genome assembly, now-a-days makes it even possible for researchers to individually sequence the genome of their choosing organisms. […]