Next Generation Sequencing

Application of NGS in PCOS Study: Polycystic ovary syndrome (PCOS) is a highly complex endocrine disorder in reproductive females, which leads to reproductive, metabolic and hormonal abnormalities and manifest a host of ovarian defects like impaired folliculogenesis, anovulation, and poor oocyte quality. Addressing the epigenetic anomalies that regulate adverse events is of foremost importance in […]

  NGS is a low-priced, speedy and high-throughput alternative to Sanger-method based DNA sequencing. It offers parallel-sequencing of millions of DNA fragments concurrently and allows an entire genome to be sequenced in a small time. NGS is basically a three-step process, namely: 1). Template preparation 2). Sequencing and imaging 3). Data analysis. All the platforms […]

Since the beginning of the Human Genome Project, data management has been perceived as a key challenge for current molecular biology research. Before the finish of the nineties, advances had been set up that adequately bolstered most continuous activities, ordinarily based upon relational database management frameworks. Ongoing years have seen a sensational increment in the […]

Genome-wide examinations concerning cooperative interactions among genomic functions, e.g. DNA replication, isolation, translation, repair and rearrangement, are fundamental for methodically explaining every single biological activity on the genome. To this end, chromatin immunoprecipitation pursued by sequencing (ChIP-seq) examination was produced to comprehend the collaboration and cooperations that happen in a wide assortment of living beings […]

With the recent advancement in DNA sequencing technologies, researches have been fortified to high-throughput, cost-efficient sequencing of many more genomes that was ever previously possible. The most prominent area of genomic sequencing and assembly, known as de-novo genome assembly, now-a-days makes it even possible for researchers to individually sequence the genome of their choosing organisms. […]

With the advancements in Sanger sequencing continuing, it has become an absolute key to enigmatic method in identification of closed closets in the gene pool. The major drawback even with the advance Sanger sequencers was the cost per base and problems related to cloning and sequencing of regions containing repetitive sequences. But with the advancement […]

Decoding DNA constituted within the human genome has been widely anticipated for the contribution it will make toward understanding human evolution, the causation of disease, and the interplay between the environment and heredity in defining the human condition. Fast progress in DNA sequencing technology has made a substantial leap towards understanding human conditions at genomic […]